Fig. 4.

Ectopic lignification in cellulose-deficient mutants does not contribute to Fo5176 resistance. A Representative images of lignin deposition visualized by phloroglucinol stain (fuchsia) in WT (Col-0) and ctl1-2 mock and Fo5176-infected roots at 1, 4, and 5 days post-treatment (dpt) with Fo5176 pSIX1::GFP microconidia. A minimum of 10 plants per genotype from at least 3 independent experiments were observed per dpt with similar results. B Schematic representation of the lignin biosynthesis pathway with focus on the bifurcation between phenylpropanoids and salicylic acid synthesis pathways. Mutants of biosynthesis enzymes in colored text are used in this study (see (C)). Figure adapted from [67]. C Root vascular penetration of WT (Col-0) and lignin-deficient mutants at various days post-treatment (dpt) with Fo5176 pSIX1::GFP microconidia. Values represent the mean ± standard error of at least 3 independent experiments, each one containing at least 13 seedlings. Statistical significance calculated via repeated measures two-way ANOVA with Tukey post-hoc test (p value ≤ 0.05 (genotype), p value ≤ 0.05 (time), p value ≤ 0.05 (genotype x time)). Significant differences at the last time point shown (7 dpt) are indicated on the graph using letters; statistics of remaining time points summarized in Additional file 9: Table S4A-F