Fig. 2.

EGFR and Src-mediated STAT3 signalling pathway is activated in TamR cells. a GSEA enrichment plots showed enrichment of the interleukin-6/Janus Kinase (JAK)/STAT3 and PI3K/Akt signalling pathways in tamoxifen-resistant breast cancer. The red horizontal bar terminating in blue indicates a shift from positively correlated genes (red) to negatively correlated genes (blue). b The protein expression levels of PI3k/Akt and MAPK/Erk signalling pathways were determined using immunoblotting. c MCF-7 and TamR cells were analysed using western blot with the indicated antibodies to determine the expression level of the EGFR and Src-mediated STAT3 signalling pathway. d The baseline levels of ERα, EGFR and STAT3 protein expression were determined by immunoblotting in TamR, MCF-7 and T47D cells. e T47D cells were incubated with tamoxifen for 24 h, 1 week, 2 weeks, 3 weeks, and 4 weeks. STAT3 protein expression levels were determined by immunoblotting. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. GAPDH was used as the loading control