Figure 1.

YAP1 and TEAD1 are highly expressed in osteosarcoma tissues and cells. (A and B) The mRNA and protein levels of YAP1 and TEAD1 were detected in osteosarcoma tissues and paired tumor-adjacent normal tissues by (A) RT-qPCR and (B) western blot analysis, respectively. *P<0.05 and **P<0.01 vs. normal tissues. (C) RT-qPCR and (D) western blot analyses were performed to determine the mRNA and protein expression levels of YAP1 and TEAD1 in normal the human osteoblast cell line NHOst and the osteosarcoma cell lines U2OS, HOS and Saos-2. All experiments were performed in triplicate. *P<0.05, **P<0.01 and ***P<0.001 vs. NHOst. YAP1, yes-associated protein 1; TEAD1, TEA domain transcription factor 1; RT-qPCR, reverse transcription-quantitative PCR.