Figure 4.

CYR61 overexpression partially reverses the inhibitory effects of VP on osteosarcoma cells. (A) RT-qPCR and (B) western blot analyses were performed to detect the mRNA and protein expression levels of the downstream targets of the Hippo signaling pathway, respectively. (C) Saos-2 cells were infected with the oeCYR61 lentivirus, and RT-qPCR and western blot analyses were conducted to determine the expression levels of CYR61 in the infected cells. (D) The effects of CYR61 overexpression on the proliferation of Saos-2 cells treated with VP were evaluated using the MTT assay. (E) Apoptosis and (F) cell cycle distribution in the oeCYR61 cells were analyzed by flow cytometry. All experiments were performed in triplicates. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 vs. Saos-2; ^##P<0.01, ^####P<0.0001 vs. Saos-2/VP. CYR61, cysteine-rich protein 61; VP, verteporfin; RT-qPCR, reverse transcription-quantitative PCR; oe, overexpression; PI, propidium iodide; PTGS2, prostaglandin-endoperoxide synthase 2; LOX, lysyl oxidase; BDNF, brain-derived neurotrophic factor; CTGF, connective tissue growth factor; DYN3, dynamin 3.