Table II.
Description of antibodies used for western blotting.
| Antibody | Target species | Host | Isotype | Clone | Dilution | Catalogue number | Manufacturer |
|---|---|---|---|---|---|---|---|
| BAFF-R | Human, mouse and rat | Mouse | IgG2b | H-1 | 1:150 | sc-365410 | Santa Cruz Biotechnology, Inc. |
| TACI | Human, mouse and rat | Mouse | IgG2b | C-9 | 1:200 | sc-365253 | Santa Cruz Biotechnology, Inc. |
| BCMA | Human | Rat | IgG2a | 335004 | 1:100 | MAB193 | R&D Systems, Inc. |
| β-actin (Direct-Blot HRP) | Human, mouse and rat | Mouse | IgG2b | 2F1-1 | 1:1000 | 643807 | BioLegend, Inc. |
| Anti-mouse IgG HRP-conjugated | Mouse | Goat | IgG | Polyclonal | 1:1000 | HAF007 | R&D Systems, Inc. |
| Anti-rat IgG HRP-conjugated | Rat | Goat | IgG | Polyclonal | 1:1000 | HAF005 | R&D Systems, Inc. |
BAFF-R, B-cell activating factor receptor; TACI, transmembrane activator and CAML interactor; BCMA, B-cell maturation antigen. HRP, horseradish peroxidase; Ig, immunoglobulin.