Observation of multi-innervated spines. A, Percentage of single- and multi-innervated spines at early, intermediate, and late phases of sLTP for sLTP and control (Ctrl) spines. B, C, 3D reconstruction of example single-innervated spine (B) and multi-innervated spine (C) with opposing presynaptic boutons and synaptic vesicles. Spines are shown in gray, PSD is shown in red, presynaptic bouton is shown in transparent blue, and synaptic vesicles are shown as golden balls. Scale bar, 500 nm.