Table 1.
Comparison of the ultrastructures of dendritic spines in control and sLTP conditions at early, intermediate and late phases of sLTP
| Feature | Early |
Intermediate |
Late |
|||
|---|---|---|---|---|---|---|
| 7 neurons from 3 batches of slicesa |
7 neurons from 5 batches of slices |
4 neurons from 3 batches of slices |
||||
| Control, n = 78 | sLTP, n = 10 | Control, n = 46 | sLTP, n = 13 | Control, n = 70 | sLTP, n = 13 | |
| Median (IQR) Mean ± SD | Median (IQR) Mean ± SD | Median (IQR) Mean ± SD | Median (IQR) Mean ± SD | Median (IQR) Mean ± SD | Median (IQR) Mean ± SD | |
| Spine head volume (µm3)b | 0.05379 (0.03476–0.07978) 0.06828 ± 0.04576 |
0.1583 (0.136–0.2999) 0.2066 ± 0.0959 |
0.04459 (0.03233–0.09994) 0.06393 ± 0.04349 |
0.0904 (0.05757–0.141) 0.1035 ± 0.0563 |
0.03414 (0.02254–0.06087) 0.04819 ± 0.04045 |
0.0461 (0.02846–0.07454) 0.0596 ± 0.0392 |
| p < 0.0001 | p = 0.0066 | p = 0.1086 | ||||
| Spine surface (µm2)b | 0.9552 (0.6714–1.422) 1.147 ± 0.6790 |
2.88 (2.149–3.346) 2.784 ± 0.7806 |
0.9292 (0.6413–1.601) 1.13 ± 0.6409 |
1.751 (1.282–2.169) 1.895 ± 0.9968 |
0.6944 (0.4661–1.072) 0.8520 ± 0.5668 |
0.8752 (0.6654–1.289) 1.03 ± 0.4711 |
| p < 0.0001 | p = 0.0012 | p = 0.0572 | ||||
| PSD (µm2)b | 0.112 (0.07733–0.1754) 0.1345 ± 0.08413 |
0.1856 (0.1486–0.246) 0.1971 ± 0.08314 |
0.07884 (0.05070–0.1571) 0.1120 ± 0.09041 |
0.103 (0.06171–0.1361) 0.09983 ± 0.04134 |
0.07141 (0.04262–0.09763) 0.08631 ± 0.07030 |
0.09868 (0.04199–0.1253) 0.116 ± 0.09073 |
| p = 0.0167 | p = 0.7213 | p = 0.1805 | ||||
| ASI (µm2)b | 0.1895 (0.1214–0.3368) 0.2348 ± 0.1632 |
0.4183 (0.3209–0.4444) 0.3845 ± 0.1307 |
0.1730 (0.1036–0.2750) 0.2166 ± 0.1525 |
0.244 (0.1975–0.3612) 0.2749 ± 0.1161 |
0.1248 (0.07355–0.1871) 0.1691 ± 0.1655 |
0.2047 (0.1504–0.259) 0.2429 ± 0.1561 |
| p = 0.0014 | p = 0.0552 | p = 0.0215 | ||||
| nsASI (µm2)b | 0.06453 (0.03304–0.1442) 0.1004 ± 0.09104 |
0.1975 (0.1527–0.2427) 0.1874 ± 0.06053 |
0.08161 (0.04831–0.1303) 0.1054 ± 0.0775 |
0.1667 (0.1144–0.2273) 0.175 ± 0.09462 |
0.04809 (0.02426–0.1054) 0.08278 ± 0.1103 |
0.1297 (0.08588–0.1543) 0.1269 ± 0.07147 |
| p < 0.0001 | p = 0.0132 | p = 0.0029 | ||||
IQR, Interquartile range.
aOne neuron per slice. One batch of slices is from one litter of pups.
bData are consistent with log-normal distribution. Welch's t test was performed on log-transformed data.