Figure 9.

TAT-CPE^452-466 blocks the interaction of TrkB and CPE in the DH and inhibits TrkB phosphorylation induced by CFC training. A, TAT-CPE^452-466 blocks the interaction between TrkB and CPE. Co-IP was performed 1 h after CFC training with microinjection of TAT-Con or TAT-CPE^452-466 into DH 30 min before CFC training with the control of home cage (HC) group. One-way ANOVA with Tukey's multiple comparisons, F_(2,9) = 15.77, ***p = 0.0009, *p = 0.0375; n = 4/group. B, TAT-CPE^452-466 inhibits the CFC-induced TrkB phosphorylation in DH. Phosphorylation and total protein levels of TrkB were assessed by Western blot 1 h after CFC training with microinjection of TAT-Con or TAT-CPE^452-466 into DH 30 min before CFC training. One-way ANOVA with Tukey's multiple comparisons, F_(2,9) = 11.86, **p = 0.0024, *p = 0.0445; n 4/group. C, Coronal sections of DG were stained with DAPI and the antibody to a pan-neuronal marker, NeuN, from the group 1 h after CFC training with microinjection of TAT-Con or TAT-CPE^452-466 into DH 30 min before CFC training. Scale bar, 20 μm. D, Spine density in basal dendrites of DG pyramidal neurons in the group 1 h after CFC training with microinjection of TAT-Con or TAT-CPE^452-466 into DH 30 min before CFC training. Scale bar, 10 μm. E, Examples and Sholl analyses of Golgi-stained dentate gyrus neurons from the group 1 h after CFC training with microinjection of TAT-Con or TAT-CPE^452-466 into DH 30 min before CFC training.