Table 15.

Independent laboratory results for spiked matrixes

Matrix	Endogenous histamine, mg/kg	Spiking, mg/kg	Total histamine mg/kg	BioSystems Y15 Histamine results
				Mean (n = 5), mg/kg	Sr	RSDr, %	Recovery, %	Bias, mg/kg
Raw tuna	1.1	0	1.1	1.1	0.3	31.0	100	0
		5	6.1	6.3	0.60	10.0	104	0.2
		10	11.1	10.6	0.60	6.0	96	–0.5
		50	51.1	51.2	0.90	1.8	100	0.1
		100	101.1	98.8	0.20	0.2	98	–2.3
		200	201.1	188.7	1.80	1.0	94	–12.3
Water-canned tuna	5.5	0	5.5	5.5	0.30	5.0	100	0
		5	10.5	10.1	0.20	1.9	96	–0.4
		10	15.5	14.4	0.50	3.4	93	–1.1
		50	55.5	50.8	0.70	1.3	92	–4.7
		100	105.5	93.7	2.60	2.7	89	–11.8
		200	205.5	176.1	1.10	0.7	86	–29.4
Oil-canned tuna	6.6	0	6.6	6.5	0.20	3.1	98	–0.1
		5	11.6	7.6	0.30	4.2	66	–3.9
		10	16.6	12.4	0.70	5.5	75	–4.1
		50	56.6	49.7	0.50	0.9	88	–6.8
		100	106.6	99.9	0.80	0.8	94	–6.6
		200	206.6	191.8	2.70	1.4	93	–14.7