TABLE 1.

ProtParam analysis of wild‐type and mutant CTSC protein

	Molecular weight (Daltons)	Theoretical pI	Instability indexa	Aliphatic index	Grand average of hydropathicity (GRAVY)b
Wild‐type	51,853.82	6.53	36.05	74.34	−0.257
Mutant	51,906.87	6.70	35.57	74.34	−0.273

^a Instability index: the instability index is >40, it predicts the protein is unstable; the instability index is <40, it predicts the protein is stable.

^b The negative value of GRAVY stands for hydrophilic protein and lower negative value represents higher hydrophilicity.