Table 2.

Measured association rates of SPON2 and FKBP5 at nuclear bodies

	SC35 speckle	RNA polymerase II factory
fa (SPON2)	99.0 %	90.5 %
fb (FKBP5)	51.0 %	93.5 %
Ntot (# in entire nucleus)	32 ± 6	403 ± 61
NIshell (# in inner four shells)	20 ± 4	170 ± 21

fa and fb are the independent association frequencies of SPON2 and FKBP5 at either SC35 speckles or RNA polymerase II factories. We took fa, fb, and the observed number of speckles or factories (N_tot) to predict co-association rate (fe, see Table 2) of the two genes at these bodies by using the formula $\text{fe = 1/}{N}_{\text{tot}}\times 1/f_{\text{a}}\times 1/f_{\text{b}}\times 4$. For the prediction of co-association in the inner four nuclear shells we used the number of bodies (N_Ishell) in that restricted sub-volume