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. 2021 Aug 18;7:71. doi: 10.1038/s41421-021-00315-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic diagram of the expression construct pPinkα-HC-RBD. P_AOX1, AOX1 promoter; SP, signal peptide; CYC1 TT, CYC1 transcription termination region. b SDS-PAGE (left panel) and western blotting (WB; right panel) analysis of purified SARS-CoV-2 RBD protein. An anti-RBD (inclusion bodies) polyclonal antibody served as the detection antibody in WB assay. c Reactivity of yeast-derived RBD with neutralizing MAbs 2H2 and 3C1 determined by ELISA. BSA served as negative control. Data shown are means ± SEM of OD450 readings from triplicate wells. d Binding affinity of yeast-derived RBD to immobilized hACE2-Fc determined by BLI. RBD protein concentrations used were shown. e Inhibition of cell entry of SARS-CoV-2 pseudovirus (PV) by yeast-produced RBD protein. Data are means ± SEM of triplicate wells. Statistical significance was determined by Student’s t-test and indicated as follows: **P < 0.01; ***P < 0.001.