Table 4.
The experimental and predicted particle size, EE% and zeta potential of the OFH-LPH, and the loading efficiency%.
| Parameter | PLGA (mg) | FH (mg) | Stirring speed (rpm) | Experimental c,d | Predicted | %Predicted error | Loading efficiency%* |
|---|---|---|---|---|---|---|---|
| Particle Size a (nm) | 5 | 20 | 1000 | 98.5 ± 3.5 | 98.2 | 0.3 | 10.3 ± 0.4% |
| EE b (%) | 65.1 ± 2.7 | 63.5 | 2.5 | ||||
| Zeta Potential a (mV) | −10.5 ± 0.45 | 10.9 | 3.8 |
a
Measured by DLS.
b
Calculated as a percentage of initial drug added, determined indirectly by HPLC.
c
Expressed as mean ± SD (n = 3).
d
Statistical analysis was carried out using a student’s T-test P < 0.05.
*
Loading efficiency % was calculated for the optimized formula only.