Fig. 3. Aβ^pH is phagocytosed by both microglia and astrocytes in situ in rat hippocampal tissue sections. (A) Schematic of phagocytosis assay in rat hippocampal tissue slices. (B) Representative 2D maximum projection of a confocal z-stack showing microglia phagocytosing Aβ^pH. Closeup of the indicated cell (yellow square) is shown on the right. Orthogonal projections at the level of the crosshairs show internalization of Aβ^pH within the microglia. (C) Representative 2D maximum projection of a confocal z-stack showing astrocytes phagocytosing Aβ^pH. Closeup of the indicated cell (yellow square) is shown on the right. Orthogonal projections at the level of the crosshairs show internalization of Aβ^pH within the astrocyte. (D) Quantification of Aβ^pH colocalized with microglia and astrocytes, as defined by IBA1⁺ and GFAP⁺ staining, respectively. Data shown as mean ± s.e.m. collected from 20 and 8 slices for microglia and astrocytes respectively (from 3 animals).