Fig. 3. (A) Confocal images of 4T1 cell incubation with DOX-Pt-tipped Au@ZIF-8 (100 μg mL−1) at different times with or without 1064 nm laser irradiation. Scale bar: 25 μm. (B) Infrared thermal images of the Au@ZIF-8, Pt-tipped Au@ZIF-8 and Pt-covered Au@ZIF-8 aqueous solutions (100 μg mL−1) irradiated by a 1 W cm−2 1064 nm laser for 5 min in vitro. (C) Fluorescence images of intracellular ROS generation from intact 4T1 cells incubated with different nanomaterials (100 μg mL−1). Scale bar: 25 μm. (D) The viability rate of 4T1 cells and HeLa cells incubated with varied concentrations of Pt-tipped Au@ZIF-8. (E) MTT viability assessment of 4T1 cells treated with different concentrations of Au@ZIF-8, Pt-covered Au@ZIF-8 and Pt-tipped Au@ZIF-8 for 24 h under 5 min of 1064 nm laser irradiation (1 W cm−2). (F) Cell viability of 4T1 cells with different treatments. (G) The fluorescence imaging of 4T1 cells stained by calcein AM (green) and PI (red) after different treatments. Scale bar: 25 μm.
