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. 2021 Jun 1;106(9):e3346–e3363. doi: 10.1210/clinem/dgab387

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — In vivo binding of STAT3, STAT5, and CREB to the second promoter in leukemia inhibitory factor (LIF)-stimulated AtT20 cells. A, DNA methylation levels in first (mPro1) and second (mPro2) promoters analyzed by bisulfite-conversion–based methylation-specific polymerase chain reaction procedure in AtT20 cells. B to D, Chromatin immunoprecipitation assay of AtT20 cells stimulated with LIF for the indicated times using B, anti-CREB (CREB); C, anti-STAT3 (STAT3); and D, anti-STAT5 (STAT5), with control antibody (immunoglobulin G [IgG]). *P < .05 vs control.