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. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: Cell Rep. 2021 Aug 3;36(5):109487. doi: 10.1016/j.celrep.2021.109487

Figure 2. Various ketogenic conditions present with global protein β-hydroxybutyrylation.

Figure 2.

(A) Left: blood β-OHB levels in mice fed control (Ctrl) or ketogenic diet (KD) for 4 weeks. n = 6, unpaired Student’s t test, ***p < 0.001. Right: western blot for pan-β-hydroxybutyryllysine (Kbhb) from liver whole-cell lysates. n = 4 replicates are quantified (right), unpaired Student’s t test, ***p < 0.001.

(B) Whole-cell lysates from Ctrl or KD conditions of the indicated tissue. IECs, intestinal epithelial cells.

(C) Type I diabetes mellitus was induced by intraperitoneal injection of vehicle (Veh) or 200 mg/kg streptozotocin (STZ). Blood measurements were taken (left) and tissue whole-cell lysates (right) were prepared 4 d post injection. n = 3 replicates for liver are quantified. Unpaired Student’s t test, **p < 0.01, ***p < 0.01.

(D) Liver whole-cell lysates from mice fed Ctrl or 10% (w/w) 1,3-butanediol (1,3-BD) diet were probed by western blot. n = 3 replicates are quantified, unpaired Student’s t test, ***p < 0.001.

Data are represented as mean ± SEM. See also Figure S2.