KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-AHCY | MBL | RN126PW |
| anti-HA | Millipore | 05–904; RRID:AB_417380 |
| anti-IgG Rb | Invitrogen | 10500C; RRID:AB_2532981 |
| anti-acetyllysine | Cell Signaling | 9441; RRID:AB_331805 |
| Anti-3-hydroxybutyryllysine | PTM Biolabs | 1201 |
| anti-light chain, HRP-conjugated | Millipore | MAB201P; RRID:AB_827270 |
| anti-p84 | GeneTex | GTX70220; RRID:AB_372637 |
| anti-α-Tubulin | Sigma | T5168; RRID:AB_477579 |
| anti-COXIV | Cell Signaling | 11967S; RRID:AB_2797784 |
| anti-AHCY | Abcam | ab134966 |
| Mouse IgG-HRP conjugate | Millipore | AP160P; RRID:AB_92531 |
| Rabbit IgG-HRP linked | Millipore | 12–348; RRID:AB_390191 |
| Chemicals, peptides, and recombinant proteins | ||
| Streptozotocin | Caymen Chemical | 13104 |
| cOmplete Protease Inhibitor Cocktail | Roche | 11697498001 |
| DL-β-hydroxybutyryl-CoA lithium salt | Sigma | H0261 |
| BioT transfection reagent | Bioland Scientific | B01–00 |
| Critical commercial assays | ||
| Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit | Biovision | K807–100 |
| Q5 Site-Directed Mutagenesis Kit | New England Biolabs | E0554S |
| (R)-(-)-3-hydroxybutyric acid sodium salt | Sigma | 298360 |
| Homocysteine ELISA Kit | BioVision | E4543–100 |
| Deposited data | ||
| Identified Kbhb peptides (see also Table S1) | ProteomeXchange | PXD016654 |
| Experimental models: Cell lines | ||
| Mouse embryonic fibroblasts (MEF) | NA | NA |
| Human embryonic kidney (HEK) 293T | NA | NA |
| Mouse hepatoma (Hepa1c1c) | NA | |
| Experimental models: Organisms/strains | ||
| C57BL/6J mouse | The Jackson Laboratory | 000664 |
| Other | ||
| Protein G Dynabeads | Thermo Fisher Scientific | 10003D |
| Normal chow diet (animal house) | Envigo, Teklad | 2020X |
| Control chow (experimental) | Envigo, Teklad | TD.150345 |
| Ketogenic diet | Envigo, Teklad | TD.160153 |
| 1,3-butanediol diet | Envigo, Teklad | TD. 160257 |
| anti-Kbhb beads | PTM Biolabs | 1204 |