Table 2.
Values of KA and Hill coefficients from FA experiments of the thrombin aptamers in different ionic environments; Tris-Glycine (TG), Tris-Glycine Potassium (TGK), Tris-Glycine Magnesium (TGM), Tris-Glycine Potassium/Magnesium (TGKM) and phosphate buffered saline (PBS). (N = 3).
| Aptamer | TG | TGK | TGM | TGKM | PBS |
|---|---|---|---|---|---|
| 15mer | KA = 25.0 ± 1.5nM n = 1.62 ± 0.16 |
KA = 51.8 ± 2.9nM n = 1.810 ± 0.090 |
KA = 30.4 ± 2.6nM n = 1.36 ± 0.11 |
KA = 23.7 ± 1.1nM n = 1.76 ± 0.13 |
KA = 29.7 ± 1.1nM n = 1.355 ± 0.047 |
| 29mer | KA = 41.5 ± 2.4nM n = 1.66 ± 0.10 |
KA = 49.2 ± 4.4nM n = 1.64 ± 0.15 |
KA = 30.5 ± 1.8nM n = 1.78 ± 0.11 |
KA = 43.1 ± 4.2nM n = 1.209 ± 0.080 |
KA = 37.0 ± 3.6nM n = 1.228 ± 0.083 |