Table 3.
A comparison of APCE and FA on the apparent affinity of the thrombin aptamers via APCE+FA hybrid assay. Values of KA and Hill coefficient for the APCE and FA portions of the hybrid assay for the 15mer and 29mer thrombin-binding aptamers. TGK was used as the assay buffer; aptamers were 5′-labeled with Texas Red.
| Aptamer | APCE | FA |
|---|---|---|
| 15mer (N = 3) | KA = 37.0 ± 2.2nM n = 1.450 ± 0.066 |
KA = 27.3 ± 1.6nM n = 1.80 ± 0.11 |
| 29mer (N = 5) | KA = 40.9 ± 1.9nM n = 1.683 ± 0.060 |
KA = 45.6 ± 2.5nM n = 1.77 ± 0.11 |