Table 5.

Summary of models used to fit APCE data for the 15mer and 29mer and the resulting binding constants. Models were fit using nonlinear least squares on Igor Pro along and all statistical values extracted from the result of the fitting.

Model	15mer			29mer
	Constant	RSD of Constant	RMS of residuals	Constant	RSD of Constant	RMS of residuals
Square Hyperbola	Kd = 44.6 ± 6.3 nM	14%	0.0485	Kd = 53 ± 12nM	23%	0.0725
Expanded Square Hyperbola	Kd = 4.5 ± 1.4nM	31%	0.0260	Kd = 4.2 ± 1.5nM	36%	0.0265
Hill Equation	KA = 37.4 ± 2.2nM n = 1.45	5.9%	0.0265	KA = 40.9 ± 1.9nM n = 1.68	4.6%	0.0215
1:2 Stepwise	Kd1 = 78 ± 14nM Kd2 = 36 ± 14nM	18% 39%	0.0218	Kd1 = 1300 ± 2100nM Kd2 = 1.3 ± 2.2nM	160% 170%	0.0114
2 Independent	Kd1 = 40 ± 370nM Kd2 = 50 ± 410nM	860% 890%	0.0486	Kd1 = 50 ± 16000nM Kd2 = 50 ± 16000nM	32000% 32000%	0.0725