Fig. 1.

Adoptive transfer of wild-type hematopoietic cells to recipients does not alter adipose tissue amount of iPk2. (A) White adipose tissue iPfk2 amount. Pfkfb3-disrupted (Pfkfb3^+/−) mice and wild-type (WT) littermates, as well as adipocyte-specific Pfkfb3-overexpressing (Adi-Tg) mice and WT littermates, were maintained on a chow diet. At 10–12 wk of age, male mice were harvested to examine iPfk2 amount in white adipose tissue (WAT) using Western blot analysis. Top panels, representative blots from WAT of 2 mice per group. Bottom panel, quantification of blots. n=4 (wild type) or 6 (Pfkfb3^+/− or Adi-Tg). (B) Pfkfb3 mRNA levels in bone marrow-derived macrophages (BMDMs) from chimeric mice. Representative images. (C) The iPfk2 amount in WAT from chimeric mice. (D) Pfkfb3 expression in WAT adipocytes and stromal cells. For B–D, male WT, Pfkfb3^+/−, and Adi-Tg mice, at 5–6 wk of age, were lethally irradiated and transplanted with bone marrow cells from WT C57BL/6J mice. After bone marrow transplantation, chimeric mice were fed a high-fat diet (HFD, 60% of fat calories) for 12 wk. After harvest, BMDMs (B) and lysates of WAT (C) were examined for Pfkfb3 mRNA levels and iPfk2 amount, respectively. Also, adipocytes and stromal cells were isolated from WAT in chimeric mice and subjected to analysis of Pfkfb3 mRNA levels (D). n=5–7. WT → WT mice, WT mice transplanted with WT hematopoietic cells; WT → Pfkfb3^+/− mice, global Pfkfb3-disrupted mice transplanted with WT hematopoietic cells; WT → Adi-Tg mice, adipocyte-specific Pfkfb3-overexpressing mice transplanted with WT hematopoietic cells. For C, top panels, representative blots for WAT lysates from 4 individual mice per group. Bottom panel, quantification of blots. n=7. For bar graphs in A, C, and D, data are means ± SEM. *, P < 0.05 and **, P < 0.01 Pfkfb3^+/− vs. WT in A or WT → Pfkfb3^+/− vs. WT → WT in C; ^†, P < 0.05 and ^††, P < 0.01 Adi-Tg vs. WT in A or WT → Adi-Tg vs. WT → WT in C and D.