Fig. 6.

Adoptive transfer of wild-type hematopoietic cells to recipients maintains the effect of aP2-driven Pfkfb3 overexpression on increasing WAT insulin signaling. WAT insulin signaling in chimeric mice. After the feeding period, chimeric mice (described in Fig. 1) were fasted for 4 h and subjected to a bolus injection of insulin (1 U/kg body weight) into the portal vein for 5 min. WAT lysates were examined for the total amount and phosphorylation states of Akt using Western blot analysis. Top panels, representative blots. Bottom panel, quantification of blots. Data are means ± SEM. n=4 (without insulin) or 6 (with insulin). ^†, P < .05 WT → Adi-Tg vs. WT → WT under insulin-stimulated condition.