Fig 4. Analysis of the vsiRNA pools shared by the RpVs.

(A) Venn diagram showing the vsiRNAs expressed in PVS and Egg stages that are shared by the permutotetraviruses. (B) Venn diagram showing the vsiRNAs expressed in PVS and Egg stages that are shared by the sobemoviruses. Only vsiRNAs with perfect match to the viral genomes were considered in these analyses. (C) Small RNA-Seq coverage of the vsiRNAs simultaneously shared by the permutotetraviruses RpV3, RpV4 and RpV7. The coverage along the consensus genome (see Methods) is displayed to highlight the accumulation of the shared vsiRNAs in the viral 3’UTR. Similar results were obtained for the RpV4 and RpV7 genomes (S7 Fig). (D) Small RNA-Seq coverage of the vsiRNAs common to the sobemoviruses RpV5 and RpV6. Sense and antisense shared vsiRNAs detected in previtellogenic stages of oogenesis (dark blue = sense, red = antisense) and mature chorionated eggs (light blue = sense, orange = antisense) are displayed.