Table 1. Oligonucleotides used in this study.
qRT-PCR | |
---|---|
Virus | Sequence (5’-3’) |
RpV1 | RpV1_1p* |
GACACAAGCTGTGATACAATC | |
RpV1_1m | |
ACTTTGGTAGACCTATAGATTC | |
RpV2 | RpV2_1p |
GAATAGAGTACGACGATCAAC | |
RpV2_1m | |
ACCGTTCGTCCGTCATCTG | |
RpV3 | RpV3_1p |
GAGACATAGTCTTGCACTTAC | |
RpV3_1m | |
TCCTGTTCATCTGCTTCTGC | |
RpV4 | RpV4_1p |
TTGCGTACCTTTGGTCAGTC | |
RpV4_1m | |
CAACTACGCTTGTTCCATGTG | |
RpV5 | RpV5_1p |
GTTCAAGCGTAGAACTATGTC | |
RpV5_1m | |
CACGAGCAAACGTGTTTGAC | |
RpV6 | RpV6_1p |
ATCATTTCCGTGTCCAGCAG | |
RpV6_1m | |
ATCATAGCAGCATAGTGCATC | |
RpV7 | RpV7_1p |
GATGATTACCGGCGCATTTTC | |
RpV7_1m | |
ATCCGTCTCCACGTGCATC | |
PCR | |
Virus | Sequence (5’-3’) |
RpV1 | RpV1_2m |
GACACAAGCTGTGATACAATC | |
RpV1_T7_1p | |
TTTTAATACGACTCACTATAGGGAGAGACACAAGCTGTGATACAATC | |
RpV1_T7_2m | |
TTTTAATACGACTCACTATAGGGAGAGACACAAGCTGTGATACAATC |
*The oligonucleotide RpV1_1p was used both in qRT-PCR and in PCR assays in combination with RpV1_1m and RpV1_2m respectively.