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. 2021 Aug 6;17(8):e1009698. doi: 10.1371/journal.pgen.1009698

Fig 4. Validation of tbx2, ufd1l and mapk8 gene disruption shows effects on the ENS in zebrafish.

Fig 4

(A) Figures and graphs showing that disruption of tbx2a/b in zebrafish does not result in overall significantly reduced numbers of phox2bb-GFP+ cells, however it does induce ENS phenotypes in category II and III in a significant number of zebrafish larvae. (B) Figures and graphs showing that disruption of ufd1l in zebrafish results in significantly reduced numbers of phox2bb-GFP+ cells and increase in the percentage of fish presenting with an ENS phenotype. (C) Figures and graphs showing that disruption of mapk8a/b in zebrafish does not result in overall significantly reduced numbers of phox2bb-GFP+ cells, however it does induce ENS phenotypes in a significant number of zebrafish larvae. Statistical tests used: students t-test and "N-1" Chi-squared test; * p < 0.05 ** p < 0.01 *** p < 0.001; Exact p-values and statistics can be found in S11 Table.