Table 1. Linearity assay of the N gene of SARS-CoV-2.

Quantity (copies/uL)	Ct N1	Ct mean	Ct SD	STD curve results
80,000	21.99	22.05	0.07	Slope: -3.28 R2: 0.994 Eff%: 101.3
20,000	23.94	23.99	0.06
5,000	25.93	26.43	0.7
1,250	28.10	28.11	0.01
312.5	30.05	30.04	0.01
78.12	31.94	31.94	0.002
Quantity (copies/uL)	Ct N2	Ct mean	Ct SD	STD curve results
80,000	23.47	23.17	0.42	Slope: -3.54 R2: 0.998 Eff%: 91.5
20,000	25.51	25.48	0.04
5,000	27.63	27.56	0.09
1,250	29.68	29.63	0.06
312.5	31.94	31.87	0.09
78.12	33.77	33.85	0.12