TABLE 1.
Comparative sensitivity and specificity of Q-PCR and visual LAMP for diagnosis of post-kala-azar dermal leishmaniasis using blood DNA at the two sites
| Site and molecular diagnostic test | Sensitivity in PKDL cases |
Specificity in controls |
||||||
|---|---|---|---|---|---|---|---|---|
| Macular |
Polymorphic |
Combined |
Ratiob | % (95% CI) | ||||
| Ratioa | % (95% CI) | Ratioa | % (95% CI) | Ratioa | % (95% CI) | |||
| NIP, ICMR | 80/80 | 100 (95.42–100) | ||||||
| Q-PCR | 26/31 | 83.87 (67.37–92.91) | 35/49 | 71.43 (49.27–75.33) | 61/80 | 76.25 (65.86–84.24) | ||
| LAMP | 24/31 | 77.42 (60.91–88.60) | 31/49 | 63.27 (49.27–75.33) | 55/80 | 68.75 (57.93–77.85) | ||
| IMS, BHU | 14/15 | 93.33 (70.18–98.91) | ||||||
| Q-PCR | 19/23 | 82.61 (62.86–93.02) | 13/17 | 76.47 (52.74–90.44) | 32/40 | 80 (65.24–89.50) | ||
| LAMP | 18/23 | 78.26 (58.10–90.34) | 12/17 | 70.59 (46.87–86.72) | 30/40 | 75 (59.81–85.81) | ||
| Combined | 94/95 | 98.95 (94.28–99.81) | ||||||
| Q-PCR | 42/54 | 83.33 (71.26–90.98) | 48/66 | 72.73 (60.96–82.0) | 93/120 | 77.50 (69.24–84.05) | ||
| LAMP | 42/54 | 77.78 (65.06–86.80) | 43/66 | 65.15 (53.11–75.52) | 85/120 | 70.83 (62.16–78.22) | ||
a
True positives/(true positives + false negatives).
b
True negatives/total negatives.