TABLE 2.
Detection of metronidazole resistance depends on addition of heme to agars
| Strain (ribotype) | MIC (μg/ml)a |
||
|---|---|---|---|
| Heme | Vitamin K1 | BHI only | |
| 17/27 (RT001)b | 16 | 1 | 1 |
| 72/25 (RT001)b | 8 | 1 | 1 |
| 7/34 (RT106)b | 8 | 1 | 1 |
| 71/01 (RT106)b | 16 | 1 | 1 |
| 70/76 (RT027)b | 8 | 1 | 1 |
| 70/75 (RT027)b | 16 | 1 | 1 |
| CD196 (RT027)c | 0.5 | 0.5 | 0.5 |
a
Effect of medium supplement on metronidazole susceptibility; supplementation of BHI agar with heme (5 μg/ml) was associated with detection of metronidazole resistance, unlike BHI supplemented with vitamin K1 (10 μg/ml) or BHI only. Results are based on three biological replicates.
b
Metronidazole resistant.
c
Metronidazole-susceptible control.