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. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: J Alzheimers Dis. 2019;72(2):575–585. doi: 10.3233/JAD-190656

Fig. 2.

Fig. 2.

Characterization of fAD EVs. A,B) Data is relative size distribution of EVs (determined by NTA, see Methods and Materials) isolated from NDC and PS1 neuronal cultures (X axis is diameter in nm). Blue numbers indicate the diameter of particles (in nm) at the various peaks. C) Isolated EVs were processed for WB and lysates probed from the EV marker flotillin-1. D–F) Relative total tau and pTau 231 levels in NDC and PS1 EVs were determined by ELISA. Data is mean tau (pg/μg of protein) (D), pTau T231 (arb. Units) (E), and pTau/Tau levels (arb. Units) (F) in lysates ± SEM. N = 4 technical replicates for NDC and 3 technical replicates for PS1. *p < 0.05; **p < 0.01; ***p < 0.001.