Table 1.
Antibodies
| Name | Epitope | Host | Source | Identifier | Dilution |
|---|---|---|---|---|---|
| AT8 | Tau phosphorylated at a.a. S202/T205 | Ms | Thermo Scientific | MN1020 | WB: 1/1000 |
| Flotillin-1 | Mouse Flotillin a.a. 312–428 | Ms | BD Biosciences | 610821 | WB: 1/200 |
| HSP 90 | N/A | Rb | Cell signaling | 4874 | WB: 1/1000 |
| K9JA (total tau) | Tau a.a. 243–441 | Rb | DAKO | A0024 | IHC: 1/1000 |
| PHF-1 | Tau phosphorylated at a.a.S396/S404 | Ms | Peter Davies | N/A | WB: 1/1000 |
| pTau(T231) | Tau phosphorylated at a.a. T231 | Rb | Abcam | ab151559 | IHC: 1/200, WB: 1/2000 |
| Tau5 (total tau) | Tau a.a. 210–241 | Ms | Millipore | MAB 361 | WB: 1/500 |
| β-actin | Sequence within the N-terminus of β-actin | Ms | Sigma | A5316 | WB: 1/2000 |