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. 2021 Jul 9;49(14):8247–8260. doi: 10.1093/nar/gkab567

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Q-modified tRNA^Tyr is preferentially imported into the mitochondria. (A) Autoradiographs showing the in vitro import of native ³²P labeled tRNA^Tyr purified from T. brucei cells grown with or without queuine. ‘No ATP’ refers to the control where ATP was not added to the reaction. ‘No mito’ refers to the negative control where nitochondria were not added. (B) Densitometric analysis of in vitro tRNA import assays from four independent experiments (P value: 0.007, Student's t test for 60 min timepoint). (C) Total RNA used to purify the native tRNAs used in the in vitro import assays, probed for tRNA^Tyr and tRNA^Glu (numbers under the blot indicate %Q levels).