Figure 4.

Distribution of mutations in the genomes of the coelimycin-producing, biosensor-positive progeny of TK24 that carry pMDE7. The complete genome sequences of TK24-YQ040, TK24-M7-BM1-1, TK24-M7-BM1-4, TK24-M7-BM2-9 and TK24-M7-BM3-3 were established in a hybrid approach using Illumina and ONT data. The resulting genomes of the pMDE7-carrying strains were compared to that of TK24-YQ040 using snippy. Lines denote mutations, with green indicating synonymous replacements, blue missense mutations, red frameshifts & stops, and black presumably neutral mutations in intergenic regions. Lines above the chromosome bars (light grey) indicate insertions, with small triangles denoting the two inserted vectors pYQ040 (green) and pMDE7 (purple). cpk cluster is indicated as an orange bar.