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. 2021 May 28;49(14):e82. doi: 10.1093/nar/gkab423

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Workflow of SCEPTRE. (1) Histone marks or other protein structures are antibody-labeled in fixed cells. (2) The sample and antibodies are linked to a swellable hydrogel grown within the sample. (3) The sample is digested by proteinase K. (4) The hydrogel is expanded in water. (5) DNA loci, alleles from the same or different genes, are labeled by FISH. (6) The sample is imaged and relevant features are extracted for analysis. (7) An epigenetic profile is constructed for each cell, comparing histone mark levels between alleles or genes.