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. 2021 Jul 7;49(14):8261–8276. doi: 10.1093/nar/gkab579

Figure 3.

Figure 3.

DDX19B tethers CTIF to the perinuclear region. (A) Immunostaining of FLAG-CTIF-WT or FLAG-CTIF-F460A. HeLa cells transiently expressing FLAG, FLAG-CTIF-WT, or FLAG-CTIF-F460A were fixed with formaldehyde and stained with α-FLAG (red) and α-lamin A/C antibodies (green). Nuclei were visualized by DAPI (blue). Representative confocal images from three biological replicates (n = 3) are provided. (B) Immunostaining of endogenous CTIF in HeLa cells depleted of endogenous DDX19B. HeLa cells were either depleted or not depleted of endogenous DDX19B. The cells were stained with α-CTIF antibody (red) and α-lamin A/C antibodies (green); n = 2. (C) Immunostaining of DDX19B-WT-HA or DDX19B-W6A/V10A-HA; n = 3. (D) In vivo bimolecular fluorescence complementation (BiFC) assay. HeLa cells were transiently co-transfected with plasmids expressing DDX19B-VN and either CTIF-WT-VC or CTIF-F460A-VC. The Venus fluorescent signal (yellow) was examined by confocal microscopy; n = 2; scale bar, 10 μm.