Anionic Metabolites (Anion Mode) Parameters

Device
Agilent CE‐TOFMS system (Agilent Technologies Inc.) machine No. 5
Capillary: fused silica capillary i.d. 50 µm × 80 cm
Analytical condition
Run buffer	Anion buffer solution (p/n: I3302‐1023)
Rinse buffer	Anion buffer solution (p/n: I3302‐1023)
Sample injection	Pressure injection 50 mbar, 22 s
CE voltage	Positive, 30 kV
MS ionization	ESI negative
MS capillary voltage	3500 V
MS scan range	m/z 50–1000
Sheath liquid	HMT sheath liquid (p/n: H3301‐1020)