Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 13;49(14):8355–8369. doi: 10.1093/nar/gkab589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — RqcP is a processivity factor essential for poly-Ala synthesis in a biochemically reconstituted RQC system. (A) Schematics of the experimental setup. Separate initiation and elongation mixtures were prepared in HEPES:Polymix buffer (7.5 Mg²⁺ pH 7.0) and incubated at 30°C for 5 min before combining. After an additional 15 minute-long incubation, tRNA species were resolved in a bis-tris gel and visualised by phosphoimaging. (B) Formation of Ala-tRNA^Ala in the reconstituted RQC system is abrogated upon omission of RqcP. (C) Addition of canonical translation elongation factor EF-Tu and 1 mM GTP does not affect the efficiency of Ala-tRNA^Ala synthesis. (D) No poly-Ala synthesis is observed either upon omission of AlaRS or in the presence of tRNA^Val, Valyl-tRNA synthetase (ValRS) and l-valine. (E) Neither RqcH^DR nor RqcP^R16A support Ala-tRNA^Ala formation in the reconstituted RQC system.