Table 2. Backbone amide (¹D_NH) RDC analysis of apo and holo Alkbh5.

Used RDCs	No. of RDCs	${\mathit{D}}_{\mathbf{a}}^{\mathbf{NH}}$ (Hz)*	η*	Rfac (%)†
Back-calculation of RDC data from x-ray structure
Apo—All RDC	184‡	8.5	0.30	47.9
Apo—Best fit	121	15.6	0.50	20.1
Zn2+/αKG—All RDC	185‡	10.4	0.32	47.7
Zn2+/αKG—Best fit	121	15.5	0.51	19.8
Zn2+/αKG/m6A—All RDC	110‡	14.0	0.63	48.0
Zn2+/αKG/m6A—Best fit	76	21.2	0.39	21.4
Ensemble refinement
Apo—All RDC l	192‡	–	–	25.8
Zn2+/αKG—All RDC	193‡	–	–	25.1
Zn2+/αKG/m6A—All RDC	116‡	–	–	25.6

*$D_{\mathrm{a}}^{\text{NH}}$ and η are the magnitude and rhombicity of the alignment tensor, respectively.

†The RDC R_fac is given by ${[\langle {(D_{\text{obs}}-D_{\text{calc}})}^2\rangle /(2\langle D_{\text{obs}}^2\rangle )]}^{1/2}$, where D_obs and D_calc are the observed and calculated RDCs, respectively (57, 58).

‡More RDCs are used in the ensemble refinement than in SVD, as the data from residues that are missing from the x-ray structure could not be used for the SVD fitting.