Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 9;49(14):8145–8160. doi: 10.1093/nar/gkab585

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Tpk1, but not other PKA components, associates with NHEJ. (A) PKA catalytic subunits, HR, NHEJ, and MRX (i.e. both NHEJ and HR) genes sharing synergistic genetic interactions (GIs; see Supplementary Table S2) with DNA damage-related genes in untreated and specific to MMS treated conditions (25,26). (B, C) Repair efficiency for plasmids (B) and HO-induced chromosomal (C; RCF, relative colony formation) DSBs on wild-type (WT) and mutant strains. Schematic (top; panel C) of the yeast JKM139 strain on chromosome III, expressing galactose-inducible HO endonuclease at MAT locus, bearing hml and hmr deletions. (D) HR efficiency of the WT and mutant strains. (E) Plasmids recovered (n ≥ 20 clones) from WT and mutant strains. Data in panels B–D are mean ± SD (n = 5 biological replicates; *P ≤ 0.05 by Student's t test).