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. 2021 Jul 19;59(8):e00859-21. doi: 10.1128/JCM.00859-21

FIG 2.

FIG 2

Example multiplex genotyping RT-qPCR amplification curves for the four hydrolysis probes: L452R (HEX), E484K (Cy5), N501Y (FAM), and N501 (Cy3.5). Horizontal lines represent each probe’s fluorescence threshold in the corresponding color. For specimens without N501Y mutations, N501 (Cy3.5) serves as an internal control in these known SARS-CoV-2-positive specimens. In addition to the single-stranded DNA (ssDNA) mutant control and wild-type whole-genome synthetic RNA control, the assay differentiates among at least five distinct genotypes depicted in this figure.