TABLE 2.
Genotyping RT-qPCR validation against whole-genome next-generation sequencing
| RT-qPCRa | Whole-genome next-generation sequencing finding |
|||||
|---|---|---|---|---|---|---|
| L452R |
E484K |
N501Y |
||||
| Detected | Not detected | Detected | Not detected | Detected | Not detected | |
| No. detected | 70 | 0 | 17 | 0 | 25 | 0 |
| No. not detected | 0 | 159 | 0 | 212 | 0 | 204 |
| PPA (95% CI)b | 100 (95–100) | 100 (80–100) | 100 (86–100) | |||
| NPA (95% CI)b | 100 (98–100) | 100 (98–100) | 100 (98–100) | |||
a
RT-qPCR relative to next-generation sequencing.
b
PPA, positive percent agreement; NPA, negative percent agreement; CI, confidence interval.