TABLE 3.
Denaturation profile analysis target oligonucleotides
| Oligonucleotidea | Sequence (5′–3′) |
|---|---|
| MAB2248 abs | CCGATCGGAGTCAACCGGTTCGCGGGCATCGAAC |
| MAB2248 bol | CCGATCGGAGTCATCCGGTTCCCGAGCATCGAAC |
| MAB2248 mas | CCGATCGGAGTCAACCGGTTCGCGGGCATCGAAC |
| MAB2830 abs | TCGATGCCTGCGCCGATGCCGGCCTCGATGCGGG |
| MAB2830 bol | TCGATGCCTGGGCCGATACCGGCCTCGATGCGGG |
| MAB2830 mas | TCGATGCCTGGACCGATACCGGCTTCGATGCGGG |
| 23S rrl 2058-2059 wt | AAGGTCCCGGGGTCTTTTCGTCCTGCCGCGCGT |
| 23S rrl 2058-2059 AG mut | AAGGTCCCGGGGTCTCTTCGTCCTGCCGCGCGT |
| 23S rrl 2058-2059 GA mut | AAGGTCCCGGGGTCTTCTCGTCCTGCCGCGCGT |
| erm(41) T28C wt | GCGGATACCAGCCCCACTGGCGTCGCGACCG |
| erm(41) T28C mt | GCGGATACCAGCCCCGCTGGCGTCGCGACCG |
| Δerm(41) wt | TGATTCCGGCCCGTAGCGTCCAATGG |
| Δerm(41) mut | TGATTCCGGCCCGGAGCGTCCAGCGG |
a
abs, M. abscessus subsp. abscessus; bol, M. abscessus subsp. bolletii; mas, M. abscessus subsp. massiliense; mut, mutant; wt, wild-type.