TABLE 4.
Interpretation of real-time PCR results for six MABC isolates
| Strains | Real-time PCR Assay Ia |
Real-time PCR Assay IIa |
Results interpretation |
|||||
|---|---|---|---|---|---|---|---|---|
|
MAB2248 bol (MB) |
23S rrl WT (MB) |
erm(41) T28C (MB) |
MAB2830 abs (MB) |
MAB2830 mas (MB) |
Δerm(41) (MB) |
Subspecies | Macrolide phenotype | |
| A | − | + | − | + | − | − | abscessus | Inducible resistance |
| B | − | + | + | + | − | − | abscessus | Susceptible |
| C | − | − | − | + | − | − | abscessus | Constitutive resistance |
| D | + | + | − | − | − | − | bolletii | Inducible resistance |
| E | − | + | − | − | + | − | massiliense | Inducible resistance |
| F | + | + | + | + | + | + | massiliense | Susceptible |
a
+, positive amplification for the molecular beacon; −, negative amplification for the molecular beacon; abs, M. abscessus subsp. abscessus; bol, M. abscessus subsp. bolletii; mas, M. abscessus subsp. massiliense; MB, molecular beacon probe; WT, wild-type.