FIG 1.

Radicicol, an Hsp90 inhibitor, strongly inhibits CHIKV infection. (A) Flow chart of the compound screening process for the identification of CHIKV antivirals. (B) The antiviral activity of radicicol was determined by dose-response curve (DRC) analysis in CHIKV-infected Vero76 cells. Cells were mock-infected (triangles) or infected with CHIKV (circles) at an MOI of 0.1 in the presence of various concentrations of radicicol. At 48 hpi, cell viability was measured using the CellTiter 96 AQueous One Solution cell proliferation assay. (C and D) Reduction of CHIKV infection by radicicol in Vero76 cells as determined by measuring infectious viral titers from cell culture supernatant and formation of viral plaques. Data represent means (± standard deviation [SD]) of at least two independent experiments performed in duplicate. Statistical analyses were determined using paired Student’s t tests, and significant differences are indicated by * (P < 0.05).