TABLE 2.
Subgroup analysis of eligible studies focused on various types of NAATa
| Diagnostic target with microbiological culture reference standard | Subgroup | NAAT | No. of data | % sensitivity (95% CI) | % specificity (95% CI) | PLR (95% CI) | NLR (95% CI) | DOR (95% CI) | AUC (95% CI) |
|---|---|---|---|---|---|---|---|---|---|
| S. aureus | In house | qPCR | 5 | 97 (93–99) | 97 (96–98) | 29.0 (11.46–73.16) | 0.04 (0.01–0.15) | 1,254.4 (159.0–9,899.5) | 99 (98–100) |
| Commercial | GeneXpert | 4 | 97 (93–100) | 93 (90–95) | 15.6 (1.88–129.1) | 0.05 (0.01–0.28) | 358.0 (43.2–2,968.3) | 99 (97–100) | |
| FA-PP | 3 | 100 (90–100) | 90 (87–93) | 9.6 (4.92–18.56) | 0.05 (0.01–0.25) | 215.4 (38.93–1,191.8) | 98 (96–99) | ||
| Unyvero PP | 3 | 81 (69–90) | 98 (96–99) | 32.6 (5.07–209.43) | 0.24 (0.08–0.74) | 158.6 (10.61–2,371.3) | 97 (96–98) | ||
| LAMP | 2 | 53 (36–69) | 94 (93–95) | 7.88 (5.36–11.57) | 0.49 (0.26–0.90) | 15.82 (7.13–35.10) | − | ||
| FA-BCID | 1 | 77 (46–95) | 100 (91–100) | − | 0.23 (0.09–0.62) | − | − | ||
| RT-qPCR | 1 | − | 93 (80–98) | − | − | − | − | ||
| SeptiFast | 1 | 60 (15–95) | 83 (71–91) | 3.44 (1.40–8.41) | 0.48 (0.16–1.43) | − | − | ||
| ELITE MGB | 1 | 99 (94–100) | 100 (88–100) | − | 0.01 (0.0–0.08) | − | − | ||
| VAPchip | 1 | 86 (64–97) | 97 (89–100) | 27.43 (6.93–108.51) | 0.15 (0.05–0.42) | − | − | ||
| MRSA | In house | qPCR | 1 | 100 (90–100) | 90 (83–95) | 9.83 (5.75–16.81) | 0.0 | ||
| Commercial | GeneXpert | 5 | 89 (82–94) | 93 (91–95) | 13.51 (6.39–28.56) | 0.1 (0.0–1,990.93) | 279.0 (45.24–1,720.5) | 99 (98–100) | |
| FA-PP | 2 | 100 (82–100) | 93 (89–95) | 11.34 (5.76–22.33) | 0.05 (0.01–0.36) | 214.63 (27.44–1,678.6) | − | ||
| LAMP | 1 | 42 (31–52) | 85 (83–86) | 2.69 (2.06–3.5) | 0.69 (0.58–0.82) | − | − | ||
| Unyvero PP | 1 | 0 (0–98) | 100 (88–100) | − | 1.0 (1.0–1.0) | − | − | ||
a
Abbreviations: −, not estimable; AUC, area under the curve; DOR, diagnostic odds ratio; FA-BCID, FilmArray blood culture identification; FA-PP, FilmArray pneumonia panel; LAMP, loop-mediated isothermal amplification; NAAT, nucleic acid amplification tests; NLR, negative likelihood ratio; PLR, positive likelihood ratio; qPCR, quantitative PCR (real-time PCR); RT-qPCR, reverse transcriptase quantitative PCR.