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EdU incorporation in replicating N. fowleri. Detection of EdU in N. fowleri trophozoites was assessed after 72 h incubation with 10 μM EdU. Images were obtained on Zeiss Elyra S1 microscope at 100×. (A) Merged channels image of EdU (green), Hoescht (blue), and Cytopainter red (red). EdU staining (B), 1 μg/ml Hoescht staining (C), and 20× Cytopainter red staining (D).
