FIG 5.
Intranasal NLP:NP immunization boosts antigen-specific CD4 T cells which localize to the lung tissue. (A) Representative plot of NP-specific CD4 T cells detected by I-Ab pMHC-II NP45 tetramer staining in LAIV and LAIV+NLP immunized murine lung. (B) Frequency of vasculature-localized CD45+ and tissue-localized CD45− pMHC-II NP45 tetramer+ cells. (C and D) Quantification of the total number of NP45-specific CD4 T cells (C) and those localized to lung tissue and vasculature (D) of LAIV and LAIV+NLP immunized mice. Results are presented as the mean number of pMHC-II NP45 tetramer+ cells per lung with standard deviation and individual replicates shown. The mean is representative of 10 individual mice analyzed as pools of two lungs each. Cell numbers from pooled lungs are divided by two to reflect the cellularity of a single lung. Significance (***, P < 0.001) for comparisons of cellular abundance between LAIV and LAIV+NLP boost cohorts was determined by two-way ANOVA with Tukey’s correction for multiple comparisons.