FIG 2.

Sarcosine replacement of 4EpDN at position 1 or 2 eliminates activity. (A) Structures of 4EpDN and its derivatives, 4EpDN 1Sar and 4EpDN 2Sar. The d-amino acid side chain is shown in red. (B) WT P. aeruginosa PA103 was grown under T3SS-inducing conditions with increasing concentrations of compounds. Secretion of T3SS cargo into the culture supernatant was assessed on SDS-PAGE gel. ExoU band intensities were visualized with Coomassie blue, quantified, and normalized to that of the DMSO control. The results are from at least two independent experiments. Error bars are standard errors of the mean.