FIG 3.

Cyclic peptomers do not affect the twin arginine translocation (Tat) system. (A) Y. pseudotuberculosis expressing a SufI-β-lactamase Tat reporter incubated in the presence of penicillin G will only grow if the Tat system remains functional. (B) Y. pseudotuberculosis SufI-β-lactamase reporters were treated with the Tat inhibitors Bay 11-7082, N-phenyl maleimide, or DMSO, and culture optical density was measured. WT refers to bacteria expressing a functional Tat secretion system. A mutant strain with a transposon insertion in the tatB gene serves as a control. (C) The same assay as in panel B was repeated in the presence of cyclic peptomers or DMSO. The result was from two independent replicates. Error bars are standard errors of the mean.