FIG 4.

Effect of cyclic peptomers on secretion of Yersinia Ysa T3SS substrates. (A and B) Y. enterocolitica serotype O:8 was grown under T3SS-inducing conditions with increasing concentrations of cyclic peptomer isomers, 4EpDN (A) and 4EpDN 2Sar (B). Secretion of T3SS cargo into the culture supernatant was assessed by precipitating secreted proteins and visualizing them with Coomassie blue. Arrow points to the YscF protein band. YspF band intensities were quantified and normalized to that of the DMSO control. Representative gel images and quantification of YspF are shown. The results are from two independent experiments. Error bars are standard errors of the mean.